Detection of clinically important beta-lactamases by using PCR

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F62157124%3A16810%2F21%3A43879646" target="_blank" >RIV/62157124:16810/21:43879646 - isvavai.cz</a>
Alternative codes found
RIV/62157124:16270/21:43879646
Result on the web
<a href="https://academic.oup.com/femsle/article-abstract/368/11/fnab068/6294906?redirectedFrom=fulltext" target="_blank" >https://academic.oup.com/femsle/article-abstract/368/11/fnab068/6294906?redirectedFrom=fulltext</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1093/femsle/fnab068" target="_blank" >10.1093/femsle/fnab068</a>

Result language
angličtina
Original language name
Detection of clinically important beta-lactamases by using PCR
Original language description
Increasing antimicrobial resistance of nosocomial pathogens is becoming a serious threat to public health. To control the spread of this resistance, it is necessary to detect beta-lactamase-producing organisms in the clinical setting. The aims of the study were to design a PCR assay for rapid detection of clinically encountered beta-lactamase genes described in Enterobacteriaceae and Gram-negative non-fermenting bacteria. The functionality of proposed primers was verified using eight reference strains and 17 strains from our collection, which contained 29 different beta-lactamase genes. PCR products of the test strains were confirmed by Sanger sequencing. Sequence analysis was performed using bioinformatics software Geneious. Overall, 67 pairs of primers for detecting 12 members of the class C beta-lactamase family, 15 members of class A beta-lactamases, six gene families of subclass B1, one member each of subclasses B2, B3 and class D beta-lactamases were designed, of which 43 pairs were experimentally tested in vitro. All 29 beta-lactamase genes, including 10 oxacillinase subgroups, were correctly identified by PCR. The proposed set of primers should be able to specifically detect 99.7% of analyzed beta-lactamase subtypes and more than 79.8% of all described beta-lactamase genes.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology

Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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