Replacement of DAPI with propidium iodide could extend the utilisation of archival tissue samples for immunofluorescent techniques

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F22%3A73617619" target="_blank" >RIV/61989592:15110/22:73617619 - isvavai.cz</a>

Result on the web

<a href="https://onlinelibrary.wiley.com/doi/10.1111/his.14676" target="_blank" >https://onlinelibrary.wiley.com/doi/10.1111/his.14676</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1111/his.14676" target="_blank" >10.1111/his.14676</a>

Result language

angličtina

Original language name

Replacement of DAPI with propidium iodide could extend the utilisation of archival tissue samples for immunofluorescent techniques

Original language description

Archival tissues are rich source of potential material for retrospective and comparative studies, which may lead to identification of novel biomarkers with prognostic and/or predictive character. Using immunofluorescence, we experienced blur, low quality of DAPI staining of nuclei in several archival samples. Because the clear recognition of nuclei is crucial in immunofluorescent techniques, we systematically investigated the effect of tissue block aging on quality of nuclear staining. In archival placental tissue samples stored for 1, 5, 18, and 26 years, DAPI staining was examined. Although DAPI is widely used in immunofluorescent staining, we tried to replaced it by propidium iodide. ImageJ software was used for recognition of nuclei. Moreover, to confirm of tissue integrity, the same samples were stained with haematoxylin. Our results showed that although the haematoxylin staining showed distinct quality in all samples, DAPI straining deteriorated with increasing age of tissue blocks and this phenomenon could be improved using propidium iodide. Replacement of DAPI by propidium iodide significantly increased detectability of cell nuclei in tissue blocks stored for 18 or 26 years by ImageJ software. Based on our results, we suppose that substitution of DAPI by propidium iodide could extend the utilization of immunofluorescent techniques to archival tissue samples.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10601 - Cell biology

Project

—

Continuities

S - Specificky vyzkum na vysokych skolach

Publication year

2022

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

HISTOPATHOLOGY

ISSN

0309-0167

e-ISSN

1365-2559

Volume of the periodical

81

Issue of the periodical within the volume

3

Country of publishing house

GB - UNITED KINGDOM

Number of pages

3

Pages from-to

409-411

UT code for WoS article

000797391000001

EID of the result in the Scopus database

2-s2.0-85129901171