Human gut bifidobacteria inhibit the growth of the opportunistic fungal pathogen Candida albicans
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F22%3A73617635" target="_blank" >RIV/61989592:15110/22:73617635 - isvavai.cz</a>
Result on the web
<a href="https://academic.oup.com/femsec/article/98/10/fiac095/6675808?login=true" target="_blank" >https://academic.oup.com/femsec/article/98/10/fiac095/6675808?login=true</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Human gut bifidobacteria inhibit the growth of the opportunistic fungal pathogen Candida albicans
Original language description
This study showed that faecal microbiota vary in their ability to inhibit the fungal pathogen Candida albicans, and identified bifidobacteria, and their fermentation acids, as inhibitory gut microbiota components.The human gut microbiota protects the host from invading pathogens and the overgrowth of indigenous opportunistic species via a process called colonization resistance. Here, we investigated the antagonistic activity of human gut bacteria towards Candida albicans, an opportunistic fungal pathogen that can cause severe infections in susceptible individuals. Coculture batch incubations of C. albicans in the presence of faecal microbiota from six healthy individuals revealed varying levels of inhibitory activity against C. albicans. 16S rRNA gene amplicon profiling of these faecal coculture bacterial communities showed that the Bifidobacteriaceae family, and Bifidobacterium adolescentis in particular, were most correlated with antagonistic activity against C. albicans. Follow-up mechanistic studies performed under anaerobic conditions confirmed that culture supernatants of Bifidobacterium species, particularly B. adolescentis, inhibited C. albicans in vitro. Fermentation acids (FA), including acetate and lactate, present in the bifidobacterial supernatants were important contributors to inhibitory activity. However, increasing the pH of both bacterial supernatants and mixtures of FA reduced their anti-Candida effects, indicating a combinatorial effect of prevailing pH and FA. This work, therefore, demonstrates potential mechanisms underpinning gut microbiome-mediated colonization resistance against C. albicans, and identifies particularly inhibitory components such as bifidobacteria and FA as targets for further study.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2022
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FEMS MICROBIOLOGY ECOLOGY
ISSN
0168-6496
e-ISSN
1574-6941
Volume of the periodical
98
Issue of the periodical within the volume
10
Country of publishing house
GB - UNITED KINGDOM
Number of pages
15
Pages from-to
"fiac095"
UT code for WoS article
000855477000001
EID of the result in the Scopus database
2-s2.0-85138458064