The kinetics of uracil-N-glycosylase distribution inside replication foci
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F25%3A73628021" target="_blank" >RIV/61989592:15110/25:73628021 - isvavai.cz</a>
Alternative codes found
RIV/00098892:_____/25:10159046
Result on the web
<a href="https://www.nature.com/articles/s41598-024-84408-x" target="_blank" >https://www.nature.com/articles/s41598-024-84408-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-024-84408-x" target="_blank" >10.1038/s41598-024-84408-x</a>
Alternative languages
Result language
angličtina
Original language name
The kinetics of uracil-N-glycosylase distribution inside replication foci
Original language description
Mismatched nucleobase uracil is commonly repaired through the base excision repair initiated by DNA uracil glycosylases. The data presented in this study strongly indicate that the nuclear uracil-N-glycosylase activity and nuclear protein content in human cell lines is highest in the S phase of the cell cycle and that its distribution kinetics partially reflect the DNA replication activity in replication foci. In this respect, the data demonstrate structural changes of the replication focus related to the uracil-N-glycosylase distribution several dozens of minutes before end of its replication. The analysis also showed that very popular synchronisation protocols based on the double thymidine block can result in changes in the UNG2 content and uracil excision rate. In response, we propose a new method for the description of the changes of the content and the activity of different cell components during cell cycle without the necessity to use synchronisation protocols.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2025
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
2045-2322
Volume of the periodical
2025
Issue of the periodical within the volume
15
Country of publishing house
GB - UNITED KINGDOM
Number of pages
19
Pages from-to
1-19
UT code for WoS article
001405676800001
EID of the result in the Scopus database
2-s2.0-85216717831