Cellular localization and metabolic function of n-butylamine induced amine oxidases in the fungus Aspergillus niger AKU 3302
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F00%3A00000973" target="_blank" >RIV/61989592:15310/00:00000973 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Cellular localization and metabolic function of n-butylamine induced amine oxidases in the fungus Aspergillus niger AKU 3302
Original language description
Using transmission electron microscopy, the amine oxidase activity in Aspergillus niger AKU 3302 was localized on the outer side of the cell wall but not inside the cell by cerium perhydroxide deposition method. Presence of cerium in the deposit was confirmed by energy dispersive microanalysis of X-rays. Interestingly, immunocytochemical localization using gold labeling with a specific antibody indicated the presence of amine oxidase protein inside the cell wall and not only on the outer surface. Besides the cell wall, high level of labeling was observed inside the cell in what seems to be secretory vesicle structures. It is proposed that the highly active amine oxidase AO-I is located in the cell wall to serve primarily as a detoxifying agent preventing amines from entering and damaging the cell. The amine oxidation exhibits an interesting spatial orientation involving a release of toxic hydrogen peroxide into extracellular space. On the other hand, the inactive amine oxidase protein
Czech name
Buněčná lokalizace a metabolické funkce aminoxidas indukovaných n-butylaminem v plísni Aspergillus niger AKU 3302
Czech description
Aktivita enzymu aminoxidasy byla pomocí transmisní elektronové mikroskopie lokalizována na vnější straně buněčné stěny. Imunocytochemická lokalizace se značenou protilátkou ukázala přítomnost neaktivního enzymu i uvnitř buňky, v sekrečních tělískách. Role aminoxidasy je diskutována v souvislosti s detoxikací - ochranou buňky před vnější přítomností toxických aminů. Přeměna aminů vede k vylučování peroxidu vodíku mimo buňku do extracelulárního prostoru. Uvnitř buňky je pravděpodobně přítomen neaktivní prekurzor enzymu.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ME%20153" target="_blank" >ME 153: Principles and applications of diverse amine oxidizing enzymes with different prosthetic groups from plants and microorganisms.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2000
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Archives of Microbiology
ISSN
0302-8933
e-ISSN
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Volume of the periodical
173
Issue of the periodical within the volume
5/6
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
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UT code for WoS article
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EID of the result in the Scopus database
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