Purification and characterization of amine oxidase and its endogenous inhibitor from Phaseolus vulgaris

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F01%3A00002026" target="_blank" >RIV/61989592:15310/01:00002026 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Purification and characterization of amine oxidase and its endogenous inhibitor from Phaseolus vulgaris
Original language description
Amine oxidase activity in the seedlings homogenates of common bean (Phaseolus vulgaris) was reported to be absent due to the presence of low-molecular endogenous inhibitor. We investigated the changes in amine oxidase activity in developing bean seedlings by the use of biosensor-immobilized tissue cuttings and by histochemical analysis. Amine oxidase was isolated and purified to apparent homogeneity from 5-days old etiolated seedlings. The isolation scheme included fractionation by ammonium sulphate, ion-exchange chromatography on DEAE-cellulose and hydrophobic chromatography on hydroxyapatite. Further purification was achieved through HPLC on ion-exchange and gel columns. Purified enzyme was characterised by native and SDS-electrophoresis. After native electrophoresis, the enzyme was transferred to cellulose acetate membranes by semi-dry blotting, but antibodies to amine oxidase from pea (Pisum sativum) did not show cross-reactivity with corresponding protein from Phaseolus. Spectrosc
Czech name
Purifikace a charakterizace aminoxidasy a jejího endogenního inhibitoru z Phaseolus vulgaris.
Czech description
Byla provedena purifikace a charakterizace aminoxidasy a jejího endogenního inhibitoru z Phaseolus vulgaris.

Project
<a href="/en/project/GA203%2F00%2FD119" target="_blank" >GA203/00/D119: Structure and properties of endogenous amine oxidase inhibitor from bean seedlings and its analogues</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2001
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Proceedings of 2nd International Symposium "Separations in the BioSciences - SBS 2001"
ISBN
80-7080-437-8
ISSN
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e-ISSN
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Number of pages
159
Pages from-to
122
Publisher name
Česká společnost chemická
Place of publication
Praha
Event location
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Event date
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Type of event by nationality
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UT code for WoS article
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