Light microscopic localization of aminoaldehyde dehydrogenase activity in plant tissues using nitroblue tetrazolium-based staining method

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F01%3A00001378" target="_blank" >RIV/61989592:15310/01:00001378 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Light microscopic localization of aminoaldehyde dehydrogenase activity in plant tissues using nitroblue tetrazolium-based staining method

Original language description

We have recently isolated pea aminoaldehydedehydrogenase(AMADH,EC1.2.1.-)and showed that it oxidises various -aminoaldehydes, but not elementary aldehydes and betaine aldehyde. Now a simple method for specific staining in polyacrylamide gels was optimised enabling us to localise AMADH activity in plant tissues. When phenazine methosulphate (PMS) was used as a mediator, AMADH in native PAGE gels readily reduced thiazolyl blue (MTT) or nitroblue tetrazolium (NBT) producing the corresponding coloured formazans. Using NBT-based staining solution, AMADH activity was localised in cross sections of the root, hypocotyl, epicotyl and shoot apex of 7-d-old etiolated pea seedlings. We followed a histochemical approach for the enzyme localisation in order to visualise tissues where the aminoaldehydes formed by the amine oxidase reaction are probably metabolised. In the root and hypocotyl, activity staining was most intense in cells belonging to the pericycle and endodermis. Weaker staining (namely

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2001

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Physiology and Biochemistry

ISSN

0981-9428

e-ISSN

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Volume of the periodical

N

Issue of the periodical within the volume

39

Country of publishing house

FR - FRANCE

Number of pages

9

Pages from-to

831-839

UT code for WoS article

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EID of the result in the Scopus database

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