Preparation of a specific anti-aminoaldehyde dehydrogenase antibody and its application for microscopic localisation of the enzyme from pea

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002056" target="_blank" >RIV/61989592:15310/04:00002056 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Preparation of a specific anti-aminoaldehyde dehydrogenase antibody and its application for microscopic localisation of the enzyme from pea

Original language description

The studied enzyme - aminoaldehyde dehydrogenase (AMADH, EC 1.2.1.19) from pea (Pisum sativum) - belongs to the group of NAD+-dependent dehydrogenases1. It catalyzes the oxidation of aminoaldehydes to the corresponding amino acids. It is involved in catabolic pathways of polyamines, which have been studied due to their regulatory properties in living organisms. Polyamines are oxidatively degraded by amine oxidases. There are two types of these enzymes: Cu-amine oxidases specific for diamines (DAO, EC 1.4.3.6.) and FAD-polyamine oxidases (PAO, EC 1.5.3.-.)2,3. Spermidine and spermine oxidation by PAO brings about the formation of 4-aminobutyraldehyde (ABAL) and N-(3-aminopropyl)-4-aminobutyraldehyde (APBAL), respectively, with the additional formation of 1,3-diaminopropane and H2O2. The diamines 1,3-diaminopropane and putrescine are converted to 3-aminopropionaldehyde (APAL) and ABAL, respectively. DAO participate in putrescine oxidation, however, the enzymatic system metabolising 1,3-d

Czech name

Příprava specifické protilátky proti aminoaldehyddehydrogenase a její použití pro mikroskopovou lokalizaci enzymu z hrachu

Czech description

Studie popisuje přípravu specifické protilátky proti aminoaldehyddehydrogenase a její použití pro mikroskopovou lokalizaci enzymu z hrachu.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Universitatis Palackianae Olomucensis, Facultas Rerum Naturalium, Chemica

ISSN

0232-0061

e-ISSN

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Volume of the periodical

43

Issue of the periodical within the volume

Suppl.

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

275

Pages from-to

50-51

UT code for WoS article

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EID of the result in the Scopus database

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