Is the degradation of cytokinins in plants assisted by phenolics?

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002097" target="_blank" >RIV/61989592:15310/04:00002097 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Is the degradation of cytokinins in plants assisted by phenolics?

Original language description

Degradation of cytokinins in plants is controlled by flavoprotein cytokinin dehydrogenase (CKX, EC 1.5.99.12). Cleavage of the cytokinin substrate proceeds via a ternary complex involving covalently bound FAD cofactor and a quinonic electron acceptor 1,2. Maize kernels 3 weeks after pollination and 2-week-old maize seedlings were examined. CKX activity was monitored using a spectrophotometric assay of the product aldehyde with 4-aminophenol3. Histochemical localization of CKX activity was done with PMS/NBT redox-dye system. Activity of laccase was stained with 3,3#-diaminobenzidine. Immunochemical detection of CKX protein was done using specific antibody against the enzyme from maize 4. CKX from maize showed the capability of using oxidation products of guaiacol, catechol, caffeic acid, acetosyringone, some flavonoids and several other compounds as electron acceptors. Further experiments revealed that the electron acceptors may be generated in vivo from plant phenolics by other enzymat

Czech name

Účastní se degradace cytokininů v rostlinách fenolové látky?

Czech description

V článku je kladena otázka, zda se účastní degradace cytokininů v rostlinách fenolové látky?

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA522%2F03%2F0979" target="_blank" >GA522/03/0979: Organization of cytokinin dehydrogenase genes in cereals and mechanism of the catalytic reaction of cytokinin degradation</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Universitatis Palackianae Olomucensis, Facultas Rerum Naturalium, Chemica

ISSN

0232-0061

e-ISSN

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Volume of the periodical

43

Issue of the periodical within the volume

S

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

275

Pages from-to

49

UT code for WoS article

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EID of the result in the Scopus database

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