Possible involvement of plant phenolics in catalytic reaction of cytokinin dehydrogenase.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002098" target="_blank" >RIV/61989592:15310/04:00002098 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Possible involvement of plant phenolics in catalytic reaction of cytokinin dehydrogenase.

Original language description

Introduction: Degradation of cytokinins in plants is controlled by flavoprotein cytokinin dehydrogenase (CKX, EC 1.5.99.12). Cleavage of the cytokinin substrate proceeds via a ternary complex involving covalently bound FAD cofactor and a quinonic electron acceptor (Bilyeu etal., 2001; Frébortová etal., 2004).Material and Methods: Maize kernels 3 weeks after pollination and 2-week-old maize seedlings were examined. CKX activity was assayed according to Frébort etal., (2002). Histochemical localization ofCKX activity was done with PMS/NBT redox-dye system. Activity of laccase was stained with 3,3#-diaminobenzidine. Immunochemical detection of CKX protein was done using specific antibody against the enzyme from maize (Morris etal., 1999).Results and Conclusions: CKX from maize showed the capability of using electron acceptors that were oxidation products of guaiacol, catechol, caffeic acid, acetosyringone, some flavonoids and several other compounds. Further experiments revealed that suc

Czech name

Fenolové látky v rostlinách se zřejmě účastní katalytické reakce cytokinindehydrogenasy

Czech description

V článku se zabýváme předpokladem, že se fenolové látky v rostlinách účastní katalytické reakce cytokinindehydrogenasy.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA522%2F03%2F0979" target="_blank" >GA522/03/0979: Organization of cytokinin dehydrogenase genes in cereals and mechanism of the catalytic reaction of cytokinin degradation</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Physiologiae Plantarum

ISSN

0137-5881

e-ISSN

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Volume of the periodical

26

Issue of the periodical within the volume

S

Country of publishing house

PL - POLAND

Number of pages

1

Pages from-to

38

UT code for WoS article

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EID of the result in the Scopus database

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