Expression of Arabidopsis thaliana cytokinin dehydrogenase in yeast Saccharomyces cerevisiae and tobacco plants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002104" target="_blank" >RIV/61989592:15310/04:00002104 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Expression of Arabidopsis thaliana cytokinin dehydrogenase in yeast Saccharomyces cerevisiae and tobacco plants

Original language description

Introduction: Cytokinin dehydrogenase (CKX, EC 1.5.99.12) controls cytokinin levels in plants by oxidative cleavage of their side chain. The amount of cytokinin dehydrogenase in plants is generally very low and its isolation and purification is thus challenging. The cloning of CKX gene from maize opened a new opportunity for study of CKX and cytokinin function in plants. The aim of this study was to prepare yeast strains for production of recombinant CKX and to characterize CKX enzymes in tobacco plantsoverexpressing AtCKXs.Material and Methods: AtCKX2 gene was cloned into pYES2 and pDR197 yeast expression plasmids or integrated into yeast genome using vectors YIplac211 and pYESX. The Saccharomyces cerevisiae strain 23344c (MAT&#61537; ura3) was used.CKX activity was assayed according to Frébort etal., (2002). Leaf extracts of wild-type and transgenic Nicotiana tabaccum L. cv. Samsun NN overexpressing Arabidopsis CKX genes 1 to 4, (Werner etal., 2001), 6 and 7, were used for characte

Czech name

Exprese cytokinindehydrogenasy z Arabidopsis thaliana v kvasince Saccharomyces cerevisiae a tabáku

Czech description

Studie je věnována expresi cytokinindehydrogenasy z Arabidopsis thaliana v kvasince Saccharomyces cerevisiae a tabáku.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

—

Project

<a href="/en/project/GA522%2F03%2F0979" target="_blank" >GA522/03/0979: Organization of cytokinin dehydrogenase genes in cereals and mechanism of the catalytic reaction of cytokinin degradation</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Physiologiae Plantarum

ISSN

0137-5881

e-ISSN

—

Volume of the periodical

26

Issue of the periodical within the volume

S

Country of publishing house

PL - POLAND

Number of pages

1

Pages from-to

37

UT code for WoS article

—

EID of the result in the Scopus database

—