Characterization of Arabidopsis cytokinin dehydrogenase expressed in yeast Saccharomyces cerevisiae and tobacco plants

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002103" target="_blank" >RIV/61989592:15310/04:00002103 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Characterization of Arabidopsis cytokinin dehydrogenase expressed in yeast Saccharomyces cerevisiae and tobacco plants

Original language description

Cytokinin dehydrogenase (CKX, EC 1.5.99.12) controls cytokinin levels in plants by oxidative cleavage of their side chain. The amount of cytokinin dehydrogenase in plants is generally very low and its isolation and purification is thus challenging. The cloning of CKX gene from maize1,2 opened a new opportunity for study of CKX and cytokinin function in plants. In Arabidopsis thaliana, seven distinct genes coding for seven proteins (AtCKX1 to AtCKX7) were found. In present study we aim to prepare yeast strains for production of recombinant AtCKX2 and to characterize CKX enzymes in tobacco plants overexpressing individual enzymes from Arabidopsis thaliana. AtCKX2 gene was cloned into pYES2 and pDR197 yeast expression plasmids or integrated into yeast genome using vectors YIplac211 and pYESX. The Saccharomyces cerevisiae strain 23344c (MAT#Á ura3) was used. CKX was secreted into the media in all tested yeast systems, the constitutive and inducible promoter driven expression showed compara

Czech name

Charakterizace cytokinindehydrogenasy z Arabidopsis thaliana exprimované v kvasince Saccharomyces cerevisiae a rostlinách tabáku

Czech description

Článek se zabývá charakterizací cytokinindehydrogenasy z Arabidopsis thaliana exprimované v kvasince Saccharomyces cerevisiae a rostlinách tabáku.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GA522%2F03%2F0979" target="_blank" >GA522/03/0979: Organization of cytokinin dehydrogenase genes in cereals and mechanism of the catalytic reaction of cytokinin degradation</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Universitatis Palackianae Olomucensis, Facultas Rerum Naturalium, Chemica

ISSN

0232-0061

e-ISSN

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Volume of the periodical

43

Issue of the periodical within the volume

S

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

2

Pages from-to

38-39

UT code for WoS article

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EID of the result in the Scopus database

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