Site-directed mutagenesis of the cytokinin dehydrogenase gene AtCKX2 from Arabidopsis thaliana

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002239" target="_blank" >RIV/61989592:15310/04:00002239 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Site-directed mutagenesis of the cytokinin dehydrogenase gene AtCKX2 from Arabidopsis thaliana

Original language description

Cell division, shoot development, growth of lateral buds, photosynthesis, chloroplast development, and delay of leaf senescence1 are processes in plants that are regulated by hormones cytokinins. A level of these hormones is regulated by their natural degradation catalyzed by the enzyme cytokinin dehydrogenase (CKX; EC 1.5.99.12). N6-chain cleavage of cytokinins2 occurs by a dehydrogenation mechanism in the active site of the CKX enzyme. Biochemical experiments show that the enzyme mediates this reaction by means of the covalently bound FAD cofactor that forms ternary complex with cytokinin substrate and electron acceptor3,4. In a majority of plants, cytokinin dehydrogenase is present in several gene forms. Multiple sequence alignment of all known CKXsreveals that they share high sequence homology in the FAD-binding and the C-terminal domains. FAD binding domain contains highly conserved N-terminal motif GHS where His is the FAD-binding residue. C-terminus of CKXs contains the motifs

Czech name

Místně cílená mutageneze cytokinindehydrogenasového genu AtCKX2 z Arabidopsis thaliana

Czech description

Byla studována místně cílená mutageneze cytokinindehydrogenasového genu AtCKX2 z Arabidopsis thaliana.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA522%2F03%2F0979" target="_blank" >GA522/03/0979: Organization of cytokinin dehydrogenase genes in cereals and mechanism of the catalytic reaction of cytokinin degradation</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Universitatis Palackianae Olomucensis, Facultas Rerum Naturalium, Chemica

ISSN

0232-0061

e-ISSN

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Volume of the periodical

43

Issue of the periodical within the volume

S

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

275

Pages from-to

257-258

UT code for WoS article

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EID of the result in the Scopus database

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