First Detection of Phytoplasmas in Rhododendron in the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002421" target="_blank" >RIV/61989592:15310/04:00002421 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

First Detection of Phytoplasmas in Rhododendron in the Czech Republic

Original language description

A 10-year-old plant of rhododendron (#Cunningham#s White# PS986) with leaf malformation and variegation was observed in Prague in 1997. This plant was micropropagated, and regenerants with severe, mild and no symptoms were obtained. Phytoplasma infectionwas detected using nested polymerase chain reaction (PCR) in the original PS986 plant and the symptomatic regenerants but not in nonsymptomatic plants. During 2002, phytoplasmas were also detected in rhododendron hybrids, PS2716 and PS2439, grown in Pruhonice that showed similar symptoms as the plant observed in Prague. In nested PCR, performed as described previously (1), primer pairs R16F1/R16R0 and R16F2/R16R2 were used. The phytoplasmas detected were classified by restriction fragment length polymorphism analysis using R16F2/R16R2 PCR fragments. Following digestion with restriction endonuclease MseI (Promega, Madison, WI), the restriction profiles obtained were identical with the pattern of the stolbur phytoplasma group (16SrXII gr

Czech name

První detekce fytoplazem u rododendronu v České Republice

Czech description

U desetiletých rostlin rhododendronu V Praze byly pozorovány malformace listů. Tyto rostliny byly mikropropagovány a byly získány regeneranti se silnými, slabými anebo žádnými příznaky. Infekcefytoplasmou byla detekována za pomocí nested-PCR v původní rostlině PS986 a v symptomatických regenerantech, nikoli v nesymptomatických rostlinách. V průběhu roku 2002 byla fytoplasma detekována také v symptomatických hybridech pěstovaných v Průhonicích. Detekované fytoplasmy blay klasifikovány pomocí RFLP, restrikční profily odpovídaly fytoplazmě stolburu (16SrXIIskupina). Podle našich informací se jedná o první nález fytoplazmy stolburu u rhodorendronu.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EE - Microbiology, virology

OECD FORD branch

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Project

<a href="/en/project/GA522%2F98%2F0792" target="_blank" >GA522/98/0792: Biological and genetic characterisation of phytoplasmas occured in the Czech</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Disease

ISSN

0191-2917

e-ISSN

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Volume of the periodical

88

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

1

Pages from-to

906-906

UT code for WoS article

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EID of the result in the Scopus database

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