Molecular Identification of Stolbur Phytoplasma Infecting Rhododendron sp.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A00003046" target="_blank" >RIV/61989592:15310/06:00003046 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Molecular Identification of Stolbur Phytoplasma Infecting Rhododendron sp.
Original language description
Ten years old Rhododendron 'Cunningham`s White' PS986 with leaf malformation and mosaic vein clearing was found in Prague in 1997. Similar symptoms were found at four years old seedling Rhododendron hybr. PS2439 on locality Průhonice in 2001. Phytoplasmainfection was detected in these plants using nested PCR 16S rDNA gene. The phytoplasmas were identified by restriction fragment length polymorphism (RFLP) analysis using amplified R16F2/R2 DNA fragments digested with restriction endonucleases RsaI, MseIand AluI. Restriction profiles of PCR products of PS986 and PS2439 plants corresponded with the stolbur phytoplasma profile (16SrXII group). Sequence analysis of the PCR products of phytoplasma isolates RHOD-CZ and RHOD1-CZ confirmed the RFLP classification.
Czech name
Molekulární detekce stolbur fytoplazmy infikující Rhododendron sp.
Czech description
Infekce fytoplazmou byla u rododendronu zjištěna pomocí nested PCR 16S rDNA genu. Fytoplazmy byly identifikovány pomocí RFLP štípáním R16F2/R2 DNA fragmentů pomocí restrikčních endonukleáz RsaI, MseI and AluI. Restrikční profily PCR produktů rostlin PS986 a PS2439 korespondují s profilem fytoplazmy stolburu (16SrXII). Sekvenční analýza PCR produktů fytoplazmových izolátů potvrdila RHOD-CZ and RHOD1-CZ RFLP klasifikaci.
Classification
Type
D - Article in proceedings
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA522%2F06%2F0618" target="_blank" >GA522/06/0618: Epidemiology of stolbur disease</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2006
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
Proceedings of the XVII. Czech and Slovak Plant Protection Conference
ISBN
80-213-1564-4
ISSN
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e-ISSN
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Number of pages
640
Pages from-to
86-91
Publisher name
Česká zemědělská univerzita
Place of publication
Praha
Event location
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Event date
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Type of event by nationality
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UT code for WoS article
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