Occurrence of Phytoplasmas in Red and White Currant in the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002424" target="_blank" >RIV/61989592:15310/04:00002424 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Occurrence of Phytoplasmas in Red and White Currant in the Czech Republic

Original language description

The presence of phytoplasmas in the shrubs of red ('Vitan', 'Losan', 'Mulka', 'Rubigo', and some of new selections) and white ('Primus') currant exhibiting full blossom symptoms was estimated using nested PCR with primers amplifying phytoplasma 16S rRNAgene and by electron microscopy. In total, 20 of 41 analysed symptomatic bushes tested positive phytoplasma in PCR. Based on the results of RFLP analysis and sequencing the phytoplasma isolates were identified as belonging to the apple proliferation andaster yellows groups. European stone fruit yellows phytoplasma was the most common phytoplasma identified in currants in this study. Furthermore, apple proliferation phytoplasma, aster yellows phytoplasma and pear decline phytoplasma were detected. The presence of phytoplasma bodies in phloem tissue of red currants was confirmed by electron microscopy.

Czech name

Výskyt fytoplasem z červeného a bílého rybízu v České republice

Czech description

Pomocí PCR a elektronové mikroskopie byla sledována přítomnost fytoplazem v kultivarech červeného a bílého rybízu, vykazujících plnokvětost. 20 ze 41 testovaných keřů bylo v PCR pozitivních. Detekované fytoplazmy byly na základě RFLP a sekvenování zařazeny do skupin fytoplazmy proliferace jabloní a fytoplazmy žloutenky aster. ESFY byla nejběžnější fytoplasmou identifikovanou ve vzorcích rybízu., nicméně byly detekovány I fytoplazma proliferace jabloní, f. žloutenky aster a f. chřadnutí hrušní.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA522%2F02%2F0040" target="_blank" >GA522/02/0040: Etiology of full blossom disease of red currant</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Horticulturae

ISSN

0567-7572

e-ISSN

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Volume of the periodical

656

Issue of the periodical within the volume

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Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

119-124

UT code for WoS article

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EID of the result in the Scopus database

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