Present Status of Research on the Etiology of the Full Blossom Disease in Red and White Currants in the Czech Republic

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002450" target="_blank" >RIV/61989592:15310/04:00002450 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Present Status of Research on the Etiology of the Full Blossom Disease in Red and White Currants in the Czech Republic

Original language description

The presence of phytoplasmas in the shrubs of red and white currants exhibiting full blossom symptoms was determined by nested PCR with primers amplifying phytoplasma 16S rRNA gene, sequencing and by electron microscopy. In total, 6 of 10 analysed symptomatic bushes gave positive PCR reaction. Based on the results of sequencing the phytoplasma isolates were identified as belonging to the apple proliferation group and aster yellows group. The presence of phytoplasma-like bodies in the phloem of two red currant bushes infected with aster yellows and apple proliferation phytoplasmas was confirmed by electron microscopy. All 10 analysed bushes were to be found infected with the Blackcurrant reversion virus. In graft experiments, the disease was transmittedfrom infected bushes to red currant ´Rondom´, ´Jonkheer van Tets´ and ´Vitan´, white currant ´Blanka´ and black currant ´Viola´. Red and white currants showed symptoms of full blossom, whereas ´Viola´ symptoms of the severe form of rever

Czech name

Současný stav výzkumu etiologie plnokvětosti u červeného a bílého rybízu v České republice.

Czech description

Pomocí PCR, sekvenování a elektronové mikroskopie byla sledována přítomnost fytoplazem v keřích červeného a bílého rybízu vykazujících plnokvětost. Na základě sekvenování byly detekováno fytoplazmy určeny jako fytoplazma žloutenky aster a fytoplazma proliferace jabloní. Tělískap odobná fytoplazmám byla identifikována I pomocí elektronové mikroskopie. U všech deset testovaných keřů byla detekována přítomnost viru zvratu rybízu. V roubovacích experimentech byl potvrzen přenos choroby na zdravé rostliny.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GA522%2F02%2F0040" target="_blank" >GA522/02/0040: Etiology of full blossom disease of red currant</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Horticulturae

ISSN

0567-7572

e-ISSN

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Volume of the periodical

656

Issue of the periodical within the volume

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Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

4

Pages from-to

99-102

UT code for WoS article

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EID of the result in the Scopus database

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