Dissecting large and complex genomes: flow sorting and BAC cloning of individual chromosomes from bread wheat

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002449" target="_blank" >RIV/61989592:15310/04:00002449 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/04:00107485

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Dissecting large and complex genomes: flow sorting and BAC cloning of individual chromosomes from bread wheat

Original language description

The analysis of the complex genome of common wheat (Triticum aestivum, 2n = 6x = 42, genome formula AABBDD) is hampered by its large size (similar to17 000 Mbp) and allohexaploid nature. In order to simplify its analysis, we developed a generic strategyfor dissecting such large and complex genomes into individual chromosomes. Chromosome 3B was successfully sorted by flow cytometry and cloned into a bacterial artificial chromosome (BAC), using only 1.8 million chromosomes and an adapted protocol developed for this purpose. The BAC library (designated as TA-3B) consists of 67 968 clones with an average insert size of 103 kb. It represents 6.2 equivalents of chromosome 3B with 100% coverage and 90% specificity as confirmed by genetic markers. This methodwas validated using other chromosomes and its broad application and usefulness in facilitating wheat genome analysis were demonstrated by target characterization of the chromosome 3B structure through cytogenetic mapping. This report on

Czech name

Rozdělení velkých genomů: třídění chromozomů pšenice a jejich klonování do vektoru BAC

Czech description

Za účelem zjednodušení analýzy genomu byla vytvořena strategie rozdělení velkých akomplexních genomů na jednotlivé chromozomy. Byla vytvořena BAC-ová knihovna 3D chromozomu ječmene. Tato zahrnuje 67 968 klonů s průměrnou velikostí inzertu 103 kb. Tato práce představuje významný skok v začlenení průtokové cytometrie do genetiky a analýzy genomů organismů.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2004

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Journal

ISSN

0960-7412

e-ISSN

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Volume of the periodical

39

Issue of the periodical within the volume

6

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

960-968

UT code for WoS article

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EID of the result in the Scopus database

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