Kinetic analysis and structural correlations of the reaction of cytokinin dehydrogenase (EC. 1.5.99.12)

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F05%3A00001818" target="_blank" >RIV/61989592:15310/05:00001818 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Kinetic analysis and structural correlations of the reaction of cytokinin dehydrogenase (EC. 1.5.99.12)

Original language description

Cytokinin dehydrogenase (CKX) is a flavoprotein that cleaves cytokinins to adenine and corresponding side-chain aldehyde using a quinone-type electron acceptor. Reactions of maize CKX with five different substrates were studied using the Stopped-flow monitoring of the reductive half-reaction revealed formation of a binary ES complex between the formed cytokinin imine and the reduced enzyme. KD values for cytokinin binding range from <2 ?M for isopentenyladenine to 66 ?M for kinetin and reasonably correspond to respective Km values. Reduction rate was high for isoprenoid cytokinins that showed specific formation of a charge transfer complex with the enzyme, but very low for other cytokinins. The ES complex decays to form a free imine-product which accumulates in the reaction mixture and only slowly hydrolyzes to adenine and the corresponding aldehyde. The imine products were identified using Q-TOF MS/MS. Mixing of the substrate-reduced enzyme with Cu2+/imidazole as an electron acceptor,

Czech name

Kinetická analýza a strukturní korelace reakce cytokinindehydrogenasy

Czech description

Cytokinindehydrogenasa (CKX) je flavoprotein, který štěpí cytokininy na adenine a aldehyd z postranního řetězce. Jednotlivé faze reakce CKX z kukuřice byly zkoumány s různými substráty pomocí spektrofotometrie zastaveného toku a byly identifikovány struktury iminových meziproduktů pomocí Q-TOF MS/MS. Kinetická data byla diskutována ve vztahu ke struktuře proteinu.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biologia Plantarum

ISSN

0006-3134

e-ISSN

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Volume of the periodical

49

Issue of the periodical within the volume

S1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

1

Pages from-to

"S3"

UT code for WoS article

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EID of the result in the Scopus database

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