Kinetic and chemical analyses of the cytokinin oxidase/dehydrogenase catalysed reaction: correlations with the crystal structure

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A00002800" target="_blank" >RIV/61989592:15310/06:00002800 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Kinetic and chemical analyses of the cytokinin oxidase/dehydrogenase catalysed reaction: correlations with the crystal structure

Original language description

CKX (cytokinin dehydrogenase) is a flavoprotein that cleaves cytokinins to adenine and the corresponding side-chain aldehyde using a quinone-type electron acceptor. In the present study, reactions of maize (Zea mays) CKX with five different substrates (N6-isopentenyladenine, trans-zeatin, kinetin, p-topolin and Nmethyl- isopentenyladenine) were studied. By using stopped-flow analysis of the reductive half-reaction, spectral intermediates were observed indicative of the transient formation of a binary enzyme-product complex between the cytokinin imine and the reduced enzyme. The reduction rate was high for isoprenoid cytokinins that showed formation of a charge-transfer complex of reduced enzyme with bound cytokinin imine. For the other cytokinins, flavin reduction was slow and no charge-transfer intermediates were observed. The binary complex of reduced enzyme and imine product intermediate decays relatively slowly to form an unbound product, cytokinin imine, which accumulates in the r

Czech name

Kinetické a chemické analýzy reakce katalyzované cytokininoxidasou/dehydrogenasou: korelace s krystalovou strukturou

Czech description

Byly studovány reakce kukuřičné cytokininoxidasy/dehydrogenasy s pěti různými substráty (N6-isopentenyladenin, trans-zeatin, kinetin, p-topolin and Nmethyl- isopentenyladenin). Pomocí metody zastaveného toku byly spektrofotometricky sledovány reakční meziprodukty. Byla prokázána tvorba přechodného binárního komplexu mezi redukovaným enzymem a iminovým derivátem substrátu. Stabilita iminových produktů umožnila jejich strukturní analýzu pomocí hmotnostní spektrometrie.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biochemical Journal

ISSN

0264-6021

e-ISSN

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Volume of the periodical

398

Issue of the periodical within the volume

Part 1

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

113-124

UT code for WoS article

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EID of the result in the Scopus database

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