High-level expression, characterization and crystal structure of Zea mays cytokinin oxidase/dehydrogenase CKO1/CKX1.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F05%3A00002161" target="_blank" >RIV/61989592:15310/05:00002161 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

High-level expression, characterization and crystal structure of Zea mays cytokinin oxidase/dehydrogenase CKO1/CKX1.

Original language description

Cytokinin oxidase/dehydrogenase (CKO/CKX) is a flavoenzyme, which irreversibly degrades cytokinins. Zea mays CKO1 was expressed in the yeast Yarrowia lipolytica. The secreted recombinant enzyme was purified using two chromatographic steps and obtained asa homogeneous protein (12 mg/l culture). ZmCKO1 exhibited a molecular mass of 70 kD, pI of 6.3 and high thermostability (T50 = 55 oC for 30 min incubation). Neutral sugar content of the molecule was 22%. Absorption and fluorescence spectra were in accordance with the presence of FAD as a cofactor. MALDI-TOF peptide mass fingerprinting correctly assigned the enzyme in MSDB protein database. Stoichiometry of aerobic CKO reaction was assessed using spectrofluorimetry. In the presence of oxygen, ZmCKO1 produces one molecule of H2O2 per one molecule of substrate. Oxidase-mode Km values of cytokinin substrates ranged from 0.5 to 1.9 &#956;M with pH optima varying between 7.3 and 8.0. The enzyme was crystallized in the presence of different i

Czech name

Vysokoúrovňová exprese a charakterizace a krystalová struktura cytokinin oxidasy/dehydrogenasy CKO1/CKX1 z kukuřice (Zea mays) v Yarrowia lipolytica

Czech description

Článek je věnován vysokoúrovňové expresi, charakterizaci a krystalové struktuře cytokinin oxidasy/dehydrogenasy CKO1/CKX1 z kukuřice (Zea mays) v Yarrowia lipolytica

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2005

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biologia Plantarum

ISSN

0006-3134

e-ISSN

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Volume of the periodical

49

Issue of the periodical within the volume

S

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

1

Pages from-to

"S6"

UT code for WoS article

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EID of the result in the Scopus database

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