Probing cytokinin homeostasis in Arabidopsis thaliana by constitutively overexpressing two forms of the maize cytokinin oxidase/dehydrogenase 1 gene

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A00002797" target="_blank" >RIV/61989592:15310/06:00002797 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/06:00083205

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Probing cytokinin homeostasis in Arabidopsis thaliana by constitutively overexpressing two forms of the maize cytokinin oxidase/dehydrogenase 1 gene

Original language description

Engineering transgenic plantswith reduced cytokinin (Ck) contents is away to analyze the role of these hormones in growth and development. Cytokinin oxidase/dehydrogenase (CKO) genes are good candidates to promote Ck deficiency. They code for enzymes degrading Cks and generally belong to multigene families. Plants constitutively expressing naturally occurring CKO genes that code for secreted or vacuolar enzymes have been described.We report on Arabidopsis transgenics constitutively overexpressing the secreted native formor an engineered nonsecreted form of the maize CKO1 enzyme. Severity of phenotype symptoms (increased root system, reduced size of aerial parts and defects in seed development) was clearly correlated with the level of enzyme activity. Aerial part was especially affected in plants overexpressing the nonsecreted enzyme, even at low activity level. In all strong overexpressers, zeatin-type metabolites were highly depleted compared to isopentenyladenine-type metabolites. At

Czech name

Zkoumání homeostázy v A. thaliana konstitutivní nadměrnou expresí dvou forem genu pro kukuřičnou cytokininoxidasu/dehydrogenasu 1

Czech description

Genetickým inženýrstvím byly připraveny transgenní rostlinny A. thaliana s nadměrnou expresí genů dvou forem cytokininoxidasy/dehydrogenasy z kukuřice. Byly nalezeny fenotypové projevy transgenoze, které byly v korelaci se sníženým obsahem cytokininů v transgenních rostlinách. Snížený obsah cytokininů byl prokázán měřením extraktů pomocí hmotnostní spektrometrie. Geny pro biosyntetický enzym isopentenyltransferasu byly regulovány směrem dolů a geny pro vlastní A. thaliana byly regulovány směrem nahoru svýjimkou domnělého cytoplazmového enzymu. Pokles v obsahu cytokininů nebyl vyvážen vyšší citlivostí receptorů.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Plant Science

ISSN

0168-9452

e-ISSN

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Volume of the periodical

171

Issue of the periodical within the volume

1

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

114-122

UT code for WoS article

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EID of the result in the Scopus database

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