Safety assessment of sanguiritrin, alkaloid fraction of Macleaya cordata, in rats

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A00003049" target="_blank" >RIV/61989592:15310/06:00003049 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/06:10180

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Safety assessment of sanguiritrin, alkaloid fraction of Macleaya cordata, in rats

Original language description

The subchronic safety of sanguiritrin, a mixture of sanguinarine ( SA) and chelerythrine (CHE) quaternary benzo[c] phenanthridine alkaloids (QBA), obtained from Macleaya cordata was assessed. Rats were fed a diet containing 120 ppm sanguiritrin ( 100 ppmQBA) for 109 days. The feed consumption and the animal weight were monitored. The content of QBA in selected tissues and plasma was determined using HPLC. It was evidenced that 2% of QBA were absorbed through the GIT while 98% were excreted in the feces. In plasma, bilirubin, urea, creatinine, glomerular filtration, AST, ALT, GMT, ALP and total antioxidant capacity were determined. In liver, GSH level, lipoperoxidation products, SOD and GPx activities and total amount of cytochrome P450 were evaluated.Damage to nuclear DNA was assessed; a P-32-postlabeling assay proved that no DNA-adducts were detected in nuclear and mitochrondrial DNA in liver. No adverse effects were observed on rat organism. QBA had no influence on the gut mucosal

Czech name

Bezpečnostní hodnocení sanguiritrinu, alakloidové frakce Macleaya cordata u krys

Czech description

Byla hodnocena bezpečnosti sanguiritrinu, směsi sanguinarinových (SA) a chelerytrinových (CHE) kvarterních benzo(c)fenantridinových alkaloidů (QBA) získaných z Macleaya cordata. Krysy byly krmeny potravou obsahující 120 ppm sanguiritrinu po 109 dní. Obsah QBA ve vybraných tkáních a plazmě byl stanoven pomocí HPLC. Bylo evidentní, že 2% QBA bylo absorbováno, zatímco 98% bylo vyloučeno v exkrementech. V plazmě byl stanovován bilirubin, močovina, kreatinin, dlomerulární filtrát, AST, ALT, GMT, ALP a totální antioxidační kapacita. V játrech byly hodnoceny hladiny GSH, lipoperoxidázové produkty, SOD a GPx aktivity a celkový obsah cytochormu P450.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

<a href="/en/project/GP203%2F03%2FD237" target="_blank" >GP203/03/D237: Analysis of natural antioxidants using HPLC with coulometric detection</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Veterinární medicína

ISSN

0375-8427

e-ISSN

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Volume of the periodical

51

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

11

Pages from-to

145-155

UT code for WoS article

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EID of the result in the Scopus database

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