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Analysis of intracellular nucleotides by capillary electrophoresis - Mass spectrometry

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F06%3A00003354" target="_blank" >RIV/61989592:15310/06:00003354 - isvavai.cz</a>

  • Result on the web

  • DOI - Digital Object Identifier

Alternative languages

  • Result language

    angličtina

  • Original language name

    Analysis of intracellular nucleotides by capillary electrophoresis - Mass spectrometry

  • Original language description

    A pilot study using capillary electrophoresis with mass spectrometry for the analysis of nucleotides in human erythrocytes is presented. Erythrocytes were incubated with 5-amino-4-imidazolecarboxamide riboside in order to mimic situation in defect of purine metabolism - AICA-ribosiduria. Characteristic AICA-ribotides together with normal nucleotides were separated by capillary electrophoresis in acetate buffer ( 20 mmol/L, pH 4.4) and identified on line by mass spectrometry.

  • Czech name

    Analýza intracelulárních nukleotidů kapilární elektroforézou ve spojení s hmotnostní spektrometrií

  • Czech description

    Práce představuje pilotní studii používající kapilární elektroforézu s hmotnostní spektrometrií pro analýzu nukleotidů v lidských erytrocytech. Erytrocyty byly inkubovány s 5-amino-4-imidazolenkaroxamidribosidem za účelem napodobení situace defektu v purinovém metabolismu AICA-robodsidurie. Charakteristické AICA-ribotidy spolu s normálními nukleotidy byly separovány pomocí kapilární elektroforézy v acetátovém pufru (20 nmol/l, pH 4,4) a identifikovány online pomocí hmotnostní spektrometrie.

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    CB - Analytical chemistry, separation

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    Z - Vyzkumny zamer (s odkazem do CEZ)

Others

  • Publication year

    2006

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Nucleosides, Nucleotides, and Nucleic Acids

  • ISSN

    1525-7770

  • e-ISSN

  • Volume of the periodical

    25

  • Issue of the periodical within the volume

    9-11

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    4

  • Pages from-to

    1233-1236

  • UT code for WoS article

  • EID of the result in the Scopus database