Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and non-traditional detection techniques
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F19%3A00107222" target="_blank" >RIV/00216224:14310/19:00107222 - isvavai.cz</a>
Result on the web
<a href="https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201800367" target="_blank" >https://onlinelibrary.wiley.com/doi/full/10.1002/elps.201800367</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201800367" target="_blank" >10.1002/elps.201800367</a>
Alternative languages
Result language
angličtina
Original language name
Capillary electrophoresis-based approaches for the study of affinity interactions combined with various sensitive and non-traditional detection techniques
Original language description
Nearly all processes in living organisms are controlled and regulated by the synergy of many biomolecule interactions involving proteins, peptides, nucleic acids, nucleotides, saccharides and small molecular weight ligands. There is growing interest in understanding them, not only for the purposes of interactomics as an essential part of system biology, but also in their further elucidation in disease pathology, diagnostics and treatment. The necessity of detailed investigation of these interactions leads to the requirement of laboratory methods characterised by high efficiency and sensitivity. As a result, many instrumental approaches differing in their fundamental principles have been developed, including those based on capillary electrophoresis. Although capillary electrophoresis offers numerous advantages for such studies, it still has one serious limitation, its poor concentration sensitivity with the most commonly used detection method – ultraviolet-visible spectrometry. However, coupling capillary electrophoresis with a more sensitive detector fulfils the above-mentioned requirement. In this review, capillary electrophoresis combined with fluorescence, mass spectrometry and several non-traditional detection techniques in affinity interaction studies are summarised and discussed, together with the possibility of conducting these measurements in microchip format.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
1522-2683
Volume of the periodical
40
Issue of the periodical within the volume
5
Country of publishing house
US - UNITED STATES
Number of pages
18
Pages from-to
625-642
UT code for WoS article
000460305100004
EID of the result in the Scopus database
2-s2.0-85059838110