Classical anticytokinins do not interact with cytokinin receptors, but inhibit cyclin-dependent kinases

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F07%3A00004793" target="_blank" >RIV/61989592:15310/07:00004793 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/07:00099660 RIV/61389030:_____/07:00099660

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Classical anticytokinins do not interact with cytokinin receptors, but inhibit cyclin-dependent kinases

Original language description

Cytokinins are a class of plant hormones that regulate the cell cycle and diverse developmental and physiological processes. Several compounds have been identified that antagonise the effects of cytokinins. Based on structural similarities and competitive inhibition, it has been assumed that these anticytokinins act through a common cellular target, namely the cytokinin receptor. Here, we examined directly, the possibility that various representative classical anticytokinins inhibit the Arabidopsis cytokinin receptors CRE1/AHK4 and AHK3. We show that pyrrolo[2,3-d]pyrimidine and pyrazolo[4,3-d]pyrimidine anticytokinins do not act as competitors of cytokinins at the receptor level. Flow cytometry and microscopic analyses revealed that anticytokinins inhibit the cell cycle and cause disorganisation of the microtubular cytoskeleton and apoptosis. This is consistent with the hypothesis that they inhibit regulatory CDK enzymes. Biochemical studies demonstrated inhibition by selected anticyt

Czech name

Classical anticytokinins do not interact with cytokinin receptors, but inhibit cyclin-dependent kinases

Czech description

Cytokinins are a class of plant hormones that regulate the cell cycle and diverse developmental and physiological processes. Several compounds have been identified that antagonise the effects of cytokinins. Based on structural similarities and competitive inhibition, it has been assumed that these anticytokinins act through a common cellular target, namely the cytokinin receptor. Here, we examined directly, the possibility that various representative classical anticytokinins inhibit the Arabidopsis cytokinin receptors CRE1/AHK4 and AHK3. We show that pyrrolo[2,3-d]pyrimidine and pyrazolo[4,3-d]pyrimidine anticytokinins do not act as competitors of cytokinins at the receptor level. Flow cytometry and microscopic analyses revealed that anticytokinins inhibit the cell cycle and cause disorganisation of the microtubular cytoskeleton and apoptosis. This is consistent with the hypothesis that they inhibit regulatory CDK enzymes. Biochemical studies demonstrated inhibition by selected anticyt

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2007

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biological Chemistry

ISSN

0021-9258

e-ISSN

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Volume of the periodical

282

Issue of the periodical within the volume

19

Country of publishing house

US - UNITED STATES

Number of pages

8

Pages from-to

14356-14363

UT code for WoS article

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EID of the result in the Scopus database

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