Cytokinins in the Bryophyte Physcomitrella patens: Analyses of Activity, Distribution, and Cytokinin Oxidase/Dehydrogenase Overexpression Reveal the Role of Extracellular Cytokinins.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F07%3A00004920" target="_blank" >RIV/61989592:15310/07:00004920 - isvavai.cz</a>
Alternative codes found
RIV/61389030:_____/07:00099665
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Cytokinins in the Bryophyte Physcomitrella patens: Analyses of Activity, Distribution, and Cytokinin Oxidase/Dehydrogenase Overexpression Reveal the Role of Extracellular Cytokinins.
Original language description
Ultra-performance liquid chromatography-tandem mass spectrometry was used to establish the cytokinin profile of the bryophyte Physcomitrella patens (Hedw.) B. S. G.; of 40 analyzed cytokinins, 20 were detected. cis-Zeatin-riboside-O-glucoside, N-6-(Delta(2)-isopentenyl) adenosine-5'-monophosphate (iPRMP), and trans-zeatin-riboside-O-glucoside were the most abundant intracellular cytokinins. In addition, the aromatic cytokinins N 6-benzyladenosine (BAR), N 6-benzyladenine, meta-, and orthotopolin were detected. Unexpectedly, the most abundant extracellular cytokinin was the nucleotide iPRMP, and its identity was confirmed by quadrupole time-of-flight mass spectrometry. The effects of overexpressing a heterologous cytokinin oxidase/ dehydrogenase (CKX; EC 1.4.3.18/ 1.5.99.12) gene (AtCKX2 from Arabidopsis [ Arabidopsis thaliana]) on the intracellular and extracellular distribution of cytokinins was assessed. In cultures of CKX-transformed plants, ultra-performance liquid chromatography-t
Czech name
Cytokininy v mechu Physcomitrella patens: Analýzy aktivity, distribuce a CKX overexprese objasnující roli extracelulárních cytokininů.
Czech description
Ultra-účinná kapalinová chromatografie s hmotnostní spektrometrií byla použita pro měření cytokininového profilu v mechu Physcomitrella patens (Hedw.) B. S. G.; bylo měřeno 40 cytokininů, z nichž 20 bylo detekováno. Hladiny cytokinů jsou diskutovány ve srovnání s jejich aktivitou, biosyntézou, distribucí a CKX overexpresí.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Physiology
ISSN
0032-0889
e-ISSN
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Volume of the periodical
145
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
15
Pages from-to
786-800
UT code for WoS article
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EID of the result in the Scopus database
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