Evidence for importance of tRNA-dependent cytokinin biosynthetic pathway in the moss Physcomitrella patens
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F08%3A00005517" target="_blank" >RIV/61989592:15310/08:00005517 - isvavai.cz</a>
Alternative codes found
RIV/61389030:_____/08:00322256
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Evidence for importance of tRNA-dependent cytokinin biosynthetic pathway in the moss Physcomitrella patens
Original language description
To study cytokinin biosynthesis, we characterized a temperature-sensitive cytokinin-overproducing mutant, oveST25, of the moss Physcomitrella patens with respect to changes in cytokinin content during thermal induction in comparison to wild type. Our findings, based on combined liquid chromatography-mass spectrometry (LC-MS) analyses, show that thermoinduction caused a strong increase of extracellular N-6-(Delta(2)-isopentenyl)adenine (iP), N-6-(Delta(2)-isopentenyl)adenosine (iPR), cis-zeatin (cZ), cis-zeatin riboside (cZR) and its O-glucoside cZROG in oveST25. In contrast, no significant changes were measured in the wild type. To investigate the relevance of tRNA for cytokinin production in Physcomitrella, we determined cytokinins in tissue and culture medium as well as in tRNA hydrolysates. The analysis of cytokinins from whole-culture extracts of wild type revealed 56% of iP-type, 32% of cZ-type, and 11% of trans-zeatin (tZ)-type forms. In tRNA, 90% of cytokinins were represented b
Czech name
Důkaz důležitosti tRNA-závislé cytokininové biosyntetické dráhy v mechu Physcomitrella patens
Czech description
Byla studována biosyntéza cytokininů pomocí teplotně-senzitivního cytokinin-overprodukujícího mutantu, oveST25, z mechu Physcomitrella patens s ohledem na změny v hladinách cytokininů během teplotní indukce v porovnání s divokým typem. Analýza cytoninů byla provedena ve tkáních i médiu a také v hydrolyzátech tRNA. Výsledky stanovení byly vzájemně porovnávány a diskutovány.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2008
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Plant Growth Regulation
ISSN
0721-7595
e-ISSN
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Volume of the periodical
27
Issue of the periodical within the volume
3
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
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UT code for WoS article
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EID of the result in the Scopus database
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