Apoplastic and non-secreted cytokinin dehydrogenase from maize: homology and diversity

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F08%3A00005616" target="_blank" >RIV/61989592:15310/08:00005616 - isvavai.cz</a>

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Apoplastic and non-secreted cytokinin dehydrogenase from maize: homology and diversity

Original language description

Plant hormones cytokinins are degraded by a cytokinin dehydrogenase (CKX, EC 1.5.99.12). There are several isoforms of CKX in each plant encoded by a small highly homologous gene family. These isoforms are differentially targeted in plant cells to achieve specific regulatory functions. Differences in local environments affect CKX activity and dictate differences in modes of biochemical action on cytokinin substrates and electron acceptors. Most of the CKX isoforms are localized in apoplast or vacuole; there is generally only one CKX isoform per plant per species that lacks a translocation signal and presumably functions in cytoplasm. The best characterized CKX from maize is ZmCKX1, known to be secreted into the apoplast, while a gene encoding the non-secreted CKX has not been cloned before. The aims of this work were to clone the gene for non-secreted CKX from maize (nsZmCKX), compare its localization with the apoplastic ZmCKX1 and characterize its biochemical and structural properties

Czech name

Apoplastická a nesekretovaná cytokinindehydrogenasa z kukuřice: homologie a diverzita

Czech description

Byl klonován gen pro cytosolickou verzi cytokinin dehydrogenasy, následně byl funkčně popsán. Byla potvrzena jeho lokalizace a srovnána s lokalizaci apoplastického enzymu ZmCKX1 pomocí konfokální mikroskopie. Bylo použito dvou fluorescenčních proteinových značek DsRed a GFP. Pro další biochemickou charakterizaci bude použito rekombinačních proteinů.

Type

D - Article in proceedings

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2008

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Studentská vědecká soutěž "O cenu děkana 2008" . Sborník příspěvků.

ISBN

978-80-244-2115-5

ISSN

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e-ISSN

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Number of pages

7

Pages from-to

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Publisher name

Univerzita Palackého

Place of publication

Olomouc

Event location

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Event date

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Type of event by nationality

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UT code for WoS article

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