Subcellular localization and biochemical comparison of cytosolic and secreted cytokinin dehydrogenase enzymes from maize

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010322" target="_blank" >RIV/61989592:15310/09:00010322 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Subcellular localization and biochemical comparison of cytosolic and secreted cytokinin dehydrogenase enzymes from maize
Original language description
The aim was to characterize the maize non-secreted CKX gene and elucidate the ZmCKX10 subcellular localization. For determination of subcellular localization, the CKX genes were fused with green fluorescent protein (GFP) and overexpressed in tomato hairyroots. Using confocal microscopy, ZmCKX1-GFP signal was confirmed to be present in the apoplast, whereas ZmCKX10-GFP was detected in the cytosol. While roots overexpressing ZmCKX1-GFP formed significantly more mass in comparison to the control, non-secreted CKX overexpression resulted in a small reduction in root mass accumulation. Biochemical characterization of ZmCKX10 was performed using recombinant protein produced in Pichia pastoris. In contrast to the preference for 2,6-dichlorophenolindophenol (DCPIP) as an electron acceptor and transzeatin, N6-(?2-isopentenyl)adenine (iP) and N6-(? 2-isopentenyl)adenosin (iPR) as substrates for ZmCKX1, the non-secreted ZmCKX10 had a range of suitable electron acceptors and different substrate p
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
<a href="/en/project/ME%20861" target="_blank" >ME 861: Biochemical and structural comparisons of two differentially targeted cytokinin dehydrogenase enzymes</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2009
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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