Vacuolar and cytosolic cytokinin dehydrogenases of Arabidopsis thaliana: heterologous expression, purification and properties

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F10%3A10212862" target="_blank" >RIV/61989592:15310/10:10212862 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/10:00359304

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Vacuolar and cytosolic cytokinin dehydrogenases of Arabidopsis thaliana: heterologous expression, purification and properties

Original language description

Cytokinin dehydrogenases (CKXs) also show low cytokinin oxidase activity, but molecular oxygen is a comparatively poor electron acceptor. This study presents the purification and characterization of non-secreted CKX enzymes and substrate specificities are discussed with respect to their compartmentation. Vacuolar enzymes AtCKX1 and AtCKX3 were produced in Pichia pastoris and cytosolic enzyme AtCKX7 was expressed in Escherichia coli. All enzymes preferred synthetic electron acceptors over oxygen, namelypotassium ferricyanide and 2,3-dimetoxy-5-methyl-1,4-benzoquinone (Q0). In slightly acidic conditions (pH 5.0), N6-(2-isopentenyl)adenine 9-glucoside (iP9G) was the best substrate for AtCKX1 and AtCKX7, whereas AtCKX3 preferentially degraded N6-(2-isopentenyl)adenine 9-riboside-5?-monophosphate (iPMP). Moreover, vacuolar AtCKX enzymes in certain conditions degraded N6-(2-isopentenyl)adenine di- and triphosphates two to five times more effectively than its monophosphate

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Phytochemistry

ISSN

0031-9422

e-ISSN

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Volume of the periodical

71

Issue of the periodical within the volume

17

Country of publishing house

GB - UNITED KINGDOM

Number of pages

9

Pages from-to

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UT code for WoS article

000285325500004

EID of the result in the Scopus database

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