Identification and quantification of several mammalian steroid hormones in plants by UPLC-MS/MS.

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010048" target="_blank" >RIV/61989592:15310/09:00010048 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/09:00328735 RIV/61388963:_____/09:00328735

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Identification and quantification of several mammalian steroid hormones in plants by UPLC-MS/MS.

Original language description

Abstract We have developed an effective method for the isolation, identification, and quantification of several mammalian steroid hormones and their metabolites in different plant tissues. The purification protocol was based on solid-phase extraction (SPE) combined with immunoaffinity chromatography (IAC) using immobilized generic polyclonal anti-D4-3-keto-steroid antibodies covalently bound to Affi-Gel 10 sorbent. The antibodies were characterized by means of enzyme-linked immunosorbent assay (ELISA).The detection limit of the ELISA was 6.0x10e-10 mol/L and cross-reactivity with most D4-3-keto-steroids was very high as predicted (68?122%). The IAC allowed fast, single-step purification of different plant extracts prior to analysis by ultra-performance liquid chromatography-electrospray tandem mass spektrometry [UPLC-ESI(+)-MS/MS]. In multiple-reaction-monitoring (MRM) mode, the detection limit of the method for most of the steroids analyzed was close to 10 fmol and the response was l

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EF - Botany

OECD FORD branch

—

Project

<a href="/en/project/KAN200380801" target="_blank" >KAN200380801: Immunonanotechnologies for hormone diagnostic</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Plant Growth Regulation

ISSN

0721-7595

e-ISSN

—

Volume of the periodical

28

Issue of the periodical within the volume

2

Country of publishing house

DE - GERMANY

Number of pages

12

Pages from-to

—

UT code for WoS article

—

EID of the result in the Scopus database

—