Immunoaffinity chromatography of abscisic acid combined with electrospray liquid chromatography?mass spectrometry.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F07%3A00004919" target="_blank" >RIV/61989592:15310/07:00004919 - isvavai.cz</a>
Alternative codes found
RIV/61389030:_____/07:00305972
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Immunoaffinity chromatography of abscisic acid combined with electrospray liquid chromatography?mass spectrometry.
Original language description
Polyclonal antibodies with high specificity for C1-immobilised (+)-cis,trans-abscisic acid (ABA) were raised, characterised by enzyme-linked immunosorbent assay (ELISA) and used for preparation of an immunoaffinity chromatography (IAC) gel. The detectionlimit of the ELISA was approximately 4.6x10-10 mol/L. Sensitive electrospray liquid chromatography?mass spectrometry (LC?ESI-MS) methods were also developed with detection limits below 0.1x10e-12 mol. The IAC allowed quick, single-step processing of samples prior to the analyses. The LC?ESI-MS and LC?ELISA techniques were used for comparative estimation of endogenous ABA levels in immunoaffinity purified extracts of normal and water-stressed Nicotiana tabacum L. leaves. The analytical approaches were validated using deuterium- and tritium-labelled internal standards, respectively. The IAC method was found to be highly effective, sensitive and convenient for isolating the target analyte from plant material.
Czech name
Imunoafinitní chromatografie kyseliny abscisové kombinovaná s kapalinovou chromotatografií-hmotnostní spektrometrií.
Czech description
Připravené vysoce specifické polyklonální protilátky proti (+)-cis,trans-abscisic acid (ABA) byly ocharakterizovány a použity pro přípravu imunoafinitního gelu. Detekční limit při stanovení v ELISA testu byl 4.6x10e-10 mol/L. V kombinaci s kapalinovou chromotatografií-hmotnostní spektrometrií bylo dosaženo limity detekce 0.1x10-12 mol. Celá metoda byla validována použitím 2H a 3H interních standardů a vyvinuté postupy byly použity při stanovení endogenních hladin ABA v listech u norálních a stresovanýchrostlin Nicotiana tabacum L.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
ISSN
1570-0232
e-ISSN
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Volume of the periodical
847
Issue of the periodical within the volume
2
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
12
Pages from-to
162-173
UT code for WoS article
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EID of the result in the Scopus database
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