Human Fbh1 helicase contributes to genome maintenance via pro- and anti-recombinase activities

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F09%3A00010371" target="_blank" >RIV/61989592:15310/09:00010371 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15110/09:00009747

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Human Fbh1 helicase contributes to genome maintenance via pro- and anti-recombinase activities

Original language description

Homologous recombination (HR) is essential for faithful repair of DNA lesions yet must be kept in check, as unrestrained HR may compromise genome integrity and lead to premature aging or cancer. To limit unscheduled HR, cells possess DNA helicases capable of preventing excessive recombination. In this study, we show that the human Fbh1 (hFbh1) helicase accumulates at sites of DNA damage or replication stress in a manner dependent fully on its helicase activity and partially on its conserved F box. hFbh1interacted with singlestranded DNA (ssDNA), the formation of which was required for hFbh1 recruitment to DNA lesions. Conversely, depletion of endogenous Fbh1 or ectopic expression of helicase-deficient hFbh1 attenuated ssDNA production after replication block. Although elevated levels of hFbh1 impaired Rad51 recruitment to ssDNA and suppressed HR, its small interfering RNA?mediated depletion increased the levels of chromatin associated Rad51 and caused unscheduled sister chromatid exch

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/IAA501370902" target="_blank" >IAA501370902: Cellular responses to replication stress: Mechanisms and the role of fragile sites in genomic instability</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Cell Biology

ISSN

0021-9525

e-ISSN

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Volume of the periodical

186

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

9

Pages from-to

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UT code for WoS article

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EID of the result in the Scopus database

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