FBH1 Helicase Disrupts RAD51 Filaments in Vitro and Modulates Homologous Recombination in Mammalian Cells

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68378050%3A_____%2F13%3A00423311" target="_blank" >RIV/68378050:_____/13:00423311 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1074/jbc.M113.484493" target="_blank" >http://dx.doi.org/10.1074/jbc.M113.484493</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1074/jbc.M113.484493" target="_blank" >10.1074/jbc.M113.484493</a>

Result language
angličtina
Original language name
FBH1 Helicase Disrupts RAD51 Filaments in Vitro and Modulates Homologous Recombination in Mammalian Cells
Original language description
Background: Homologous recombination is regulated both positively and negatively in eukaryotic cells to suppress genomic instability. Results: FBH1 can disrupt RAD51 filaments in vitro and suppresses formation of spontaneous RAD51 foci in mammalian cells. In cells defective for FBH1, hyper-recombination is observed. Conclusion: FBH1 is a negative regulator of homologous recombination. Significance: RAD51 activity must be carefully controlled to preserve genomic integrity. Efficient repair of DNA doublestrand breaks and interstrand cross-links requires the homologous recombination (HR) pathway, a potentially error-free process that utilizes a homologous sequence as a repair template. A key player in HR is RAD51, the eukaryotic ortholog of bacterial RecA protein. RAD51 can polymerize on DNA to form a nucleoprotein filament that facilitates both the search for the homologous DNA sequences and the subsequent DNA strand invasion required to initiate HR. Because of its pivotal role in HR, R
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
<a href="/en/project/GAP305%2F10%2F0281" target="_blank" >GAP305/10/0281: Úloha produktu genu Rothmund-Thomsonova syndromu při údržbě genetické stability</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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