Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F10%3A10212711" target="_blank" >RIV/61989592:15310/10:10212711 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Recondensation level of repetitive sequences in the plant protoplast nucleus is limited by oxidative stress
Original language description
Protoplast cultures are remarkable examples of plant cell dedifferentiation. The state of dedifferentiation is evidenced by changes in cell morphology, genome organization, as well as by the capability of protoplasts to differentiate into multiple typesof cells. The first change in the genome structure is connected with large-scale chromatin decondensation, affecting chromocenters involving various types of these repetitive sequences. This paper describes not only the de- and re-condensation of repetitive sequences, but it also compares the recondensation level of chromatin with the levels of oxidative stress which were decreased by using an antioxidant. We have demonstrated that the treatment of protoplasts with ascorbic acid not only decreased the level of oxidative stress but also positively stimulated the expression of the ascorbate peroxidase and catalase. It also led to a greater recondensation of the chromatin (when compared to the untreated protoplasts).
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Project
<a href="/en/project/2E08018" target="_blank" >2E08018: Innovative promotion in the media of involvement women to science and research as a factor of knowledge society and equal opportunities intensification, as well as rise of competitive advantage in the case of natural sciences.</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year
2010
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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