Tandem mass spectrometry identification and LC-MS quantification of intact cytokinin nucleotides in K-562 human leukemia cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F10%3A10217256" target="_blank" >RIV/61989592:15310/10:10217256 - isvavai.cz</a>

Alternative codes found

RIV/61389030:_____/10:00351018

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Tandem mass spectrometry identification and LC-MS quantification of intact cytokinin nucleotides in K-562 human leukemia cells

Original language description

We describe here a new reversed phase high performance liquid chromatography with mass spectrometry detection method for quantifying intact cytokinin nucleotides in human K-562 leukemia cells. Tandem mass spectrometry (MS/MS) was used to identify the intracellular metabolites (cytokinin mono- di- and tri-phosphorylated nucleotides) in riboside-treated cells. For the protein precipitation and sample preparation, a trichloroacetic acid extraction method is used. Samples are then back-extracted with diethyleter, lyophilized, reconstituted and injected into the LC system. Analytes were quantified in negative selected ion monitoring (SIM) mode using a single quadrupole mass spectrometer. The method was validated in terms of retention time stabilities, limits of detection (LOD), linearity, recovery and analytical accuracy. The developed method was linear in the range of 1-1000 pmol for all studied compounds. The limits of detection for the analytes vary from 0.2 to 0.6 pmol.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2010

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

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Volume of the periodical

398

Issue of the periodical within the volume

5

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

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UT code for WoS article

000283244500028

EID of the result in the Scopus database

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