Quantitative analysis of cytokinins in plants by liquid chromatography/single-quadrupole mass spectrometry
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002219" target="_blank" >RIV/61989592:15310/04:00002219 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Quantitative analysis of cytokinins in plants by liquid chromatography/single-quadrupole mass spectrometry
Original language description
The method for estimation of 37 naturally occurring cytokinins is based on an immunoaffinity purification (IAC) and a baseline chromatographic separation of 20 cytokinins (trans/cis-zeatin, dihydrozeatin, isopentenyladenine, benzyladenine, meta/ortho-topoline groups) on reversed-phase column and analysis by high-performance liquid chromatography-mass spectrometry (HPLC/MS). When low cone voltage (20 V) was applied, all studied cytokinins were determined in aqueous methanol as dominant quasi-molecular ions of [M+H]+ with limits of detection between 10 and 50 fmol and with linearity ranges of calibration between 25 fmol and 100 pmol. The method was validated by deuterium or 15N labeled standards (isoprenoid and aromatic), added during extraction. Two different extraction procedures (70% ethanol or Bieleski buffer) have been tested. Bieleski extracts were purified by two different ion-exchange chromatography steps (SCX and DEAE Sephadex) in combination with immunoaffinity purification bas
Czech name
Kvantitavní stanovení cytokininů v rostlinách metodou kapalinové chromatografie-hmotnostní spektrometrie
Czech description
Byla studována metoda pro kvantitavní stanovení cytokininů v rostlinách metodou kapalinové chromatografie-hmotnostní spektrometrie.
Classification
Type
D - Article in proceedings
CEP classification
EF - Botany
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GA522%2F03%2F0323" target="_blank" >GA522/03/0323: Investigation of algae and cyanobacteria as producers of biologically active substances</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)
Others
Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
18th IPGSA conference, Conference Handbook
ISBN
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ISSN
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e-ISSN
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Number of pages
144
Pages from-to
124
Publisher name
Australian National University
Place of publication
Canberra
Event location
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Event date
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Type of event by nationality
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UT code for WoS article
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