Aplication of HPLC/ESI-MS for estimation of cytokinins in algae

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F04%3A00002425" target="_blank" >RIV/61989592:15310/04:00002425 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Aplication of HPLC/ESI-MS for estimation of cytokinins in algae
Original language description
The sensitive method for quantitative analysis of natural isoprenoid cytokinins (base, riboside, 9-glucoside, O-glucoside and ribotide) was developed. The method for estimation of 37 naturally occurring cytokinins is based on a baseline chromatographic separation of 20 cytokinins (trans/cis-zeatin, dihydrozeatin, isopentenyladenine, benzyladenine, meta/ortho-topoline groups). Ethanolic extracts containing a mixture of deuterium or 15N labeled standards were purified using a combined DEAE-Sephadex, octadecysilica column and immunoaffinity column based on wide-range specific monoclonal antibodies and analyzed by HPLC linked to a micromass single quadrupole mass spectrometer with an electrospray interface (HPLC/ESI-MS) and a photodiode array detector. Allstudied cytokinins were determined in aqueous methanol as dominant quasi-molecular ions of [M+H]+ with limits of detection between 10 and 50 fmol and with linearity ranges of calibration between 25 fmol and 100 pmol. Analyses of O-glucos
Czech name
Využití HPLC/ESI-MS pro stanovení cytokininů v řasách
Czech description
Byla vyvinuta citlivá metoda (HPLC/ESI-MS) pro kvantitativní analýzu přírodních isoprinoidních cytokininů v řasách.

Project
<a href="/en/project/GA522%2F03%2F0323" target="_blank" >GA522/03/0323: Investigation of algae and cyanobacteria as producers of biologically active substances</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
International Symposium on Microalgae and Seaweed products in Plant/Soil-Systems
ISBN
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ISSN
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e-ISSN
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Number of pages
50
Pages from-to
30
Publisher name
European Society of Microalgal Biotechnology
Place of publication
Mosonmagyaróvár
Event location
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Event date
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Type of event by nationality
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UT code for WoS article
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