Liquid chromatographic method for enantiopurity control of alaptide using polysaccharide stationary phases

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F11%3A33117970" target="_blank" >RIV/61989592:15310/11:33117970 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/jssc.201100135" target="_blank" >http://dx.doi.org/10.1002/jssc.201100135</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/jssc.201100135" target="_blank" >10.1002/jssc.201100135</a>

Result language

angličtina

Original language name

Liquid chromatographic method for enantiopurity control of alaptide using polysaccharide stationary phases

Original language description

Separation of veterinary drug alaptide ((S)-8-methyl-6,9-diazaspiro(4,5) decane-7,10-dione) from a chiral impurity (R-enantiomer) was developed. Five chiral columns (three amylose and two cellulose type) were evaluated in a reversed-phase system. Three of them offered satisfactory enantiomeric resolution. Finally, three methods were validated and proved to be applicable for the determination of a chiral impurity content below 0.1% (method A: 3-AmyCoat column, tris-[3,5-dimethylphenyl] carbamoyl amylose;mobile phase: water/methanol/propan-2-ol/butan-2-ol=75:20:3.5:1.5 v/v, flow rate: 0.40 mL/min; column temperature: 30 degrees C; method B: Chiralpak AS-3R, tris-[1-phenylethyl] carbamoyl amylose; water/acetonitrile=80:20 v/v, 0.40 mL/min; 40 degrees C;method C: Chiralcel OZ-3R, tris-[3-chloro-4-methylphenyl] carbamoyl cellulose; water/acetonitrile=80:20 v/v, 0.40 mL/min; 40 degrees C). Some decrease in efficiency with repeated sample injections was observed for the 3-AmyCoat column. Th

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

—

Project

<a href="/en/project/ED2.1.00%2F03.0058" target="_blank" >ED2.1.00/03.0058: Regional Centre of Advanced Technologies and Materials</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Separation Science

ISSN

1615-9306

e-ISSN

—

Volume of the periodical

34

Issue of the periodical within the volume

12

Country of publishing house

DE - GERMANY

Number of pages

5

Pages from-to

1402-1406

UT code for WoS article

000291986600006

EID of the result in the Scopus database

—