Electrochemical Determination of Transmembrane Protein Na+/K+ ATPase and Its Cytoplasmic Loop C45

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33139119" target="_blank" >RIV/61989592:15310/12:33139119 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15110/12:33139119

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Electrochemical Determination of Transmembrane Protein Na+/K+ ATPase and Its Cytoplasmic Loop C45

Original language description

Electrochemistry of membrane proteins is complicated by the fact that the studied substances are poorly soluble or insoluble in aqueous environment. The solubilization of proteins using surfactants (detergents) affects the electrochemical analysis or even renders it impossible. In the present study, the electrochemistry of the transmembrane protein Na+/K+-ATPase (NKA) and its water-soluble isolated cytoplasmic loop C45 is described. The proteins were studied using adsorptive transfer cyclic voltammetryand square-wave voltammetry on basal-plane pyrolytic graphite electrode (PGE) as well as constant-current chronopotentiometric stripping analysis on hanging mercury drop electrode (HMDE). The nonionic surfactant octaethylene glycol monododecyl ether (C12E8) was used for NKA solubilization. Under these conditions the oxidation currents of Tyr and Trp (peak Y: +0.55 V and peak W: +0.7 V, vs. Ag/ AgCl/3 M KCl) and catalytic reduction currents (peak H: 1.8 V) of NKA and C45 loop can be obser

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electroanalysis

ISSN

1040-0397

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

8

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

"1758 ? 1765"

UT code for WoS article

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EID of the result in the Scopus database

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