Haploid and mixoploid cucumber (Cucumis sativus L.) protoplasts - isolation and fusion

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33140482" target="_blank" >RIV/61989592:15310/12:33140482 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Result language
angličtina
Original language name
Haploid and mixoploid cucumber (Cucumis sativus L.) protoplasts - isolation and fusion
Original language description
This paper reports on haploid and mixoploid protoplasts in the genus Cucumis. The mixoploid plants were obtained after oryzalin treatments and the mesophyll protoplasts were isolated and cultuvated. The isolation and cultivation of the cucumber and muskmelon haploid protoplasts from young-stage pollen grains were improved. Somatic hybridization (fusion) between mixoploid cucumber protoplasts and muskmelon mesophyll and callus protoplasts by chemical fusion with polyethylene glycol (PEG, 6000) was performed for the first time. Heterofusants were observed and developed into microcolonies. Gametosomatic hybridization between mixoploid cucumber protoplasts and pollen muskmelon protoplasts was performed for the first time. heterofusants and first cell division were observed. Different ploidy, especially the mixoploid character of isolated protoplasts, has positive influence on protoplasts isolation and the following fusion.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
GE - Plant cultivation
OECD FORD branch
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Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical
Journal of Applied Botany and Food Quality-Angewandte Botanik
ISSN
1613-9216
e-ISSN
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Volume of the periodical
85
Issue of the periodical within the volume
85
Country of publishing house
DE - GERMANY
Number of pages
9
Pages from-to
64-72
UT code for WoS article
000310189700009
EID of the result in the Scopus database
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