Avidin Functionalized Maghernite Nanoparticles and Their Application for Recombinant Human Biotinyl-SERCA Purification
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33141654" target="_blank" >RIV/61989592:15310/12:33141654 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1021/la303148u" target="_blank" >http://dx.doi.org/10.1021/la303148u</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/la303148u" target="_blank" >10.1021/la303148u</a>
Alternative languages
Result language
angličtina
Original language name
Avidin Functionalized Maghernite Nanoparticles and Their Application for Recombinant Human Biotinyl-SERCA Purification
Original language description
We report on the surface characterization, functionalization, and application of stable water suspensions of novel surface active maghemite narioparticles (SAMNs), characterized by a diameter of 11 +/- 2 nm and possessing peculiar colloidal properties and surface interactions. These features permitted the acquisition of titration curves and aqueous UV-vis spectra and suggested a role played by surface under-coordinated iron atoms. This new class of nanoparticles was obtained through an easy, inexpensive, one-step, green procedure and functionalized with ligands of high biotechnological interest, such as biotin and avidin, by simple incubation in aqueous solution. Bound avidin was determined by measuring the disappearance of free avidin absorbance at 280 nm, as a function of increasing nanoparticle concentration, showing the presence of 10 +/- 3 avidin molecules per nanoparticle. The biological activity of the SAMN@avidin complex was evaluated and the number of available biotin binding
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CF - Physical chemistry and theoretical chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Langmuir: the A C S journal of surfaces and colloids
ISSN
0743-7463
e-ISSN
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Volume of the periodical
28
Issue of the periodical within the volume
43
Country of publishing house
US - UNITED STATES
Number of pages
10
Pages from-to
15392-15401
UT code for WoS article
000310484300032
EID of the result in the Scopus database
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