Construction and characterization of a reporter gene cell line for assessment of human glucocorticoid receptor activation

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33142474" target="_blank" >RIV/61989592:15310/12:33142474 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11160/12:10124917
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S0928098712003855" target="_blank" >http://www.sciencedirect.com/science/article/pii/S0928098712003855</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ejps.2012.10.003" target="_blank" >10.1016/j.ejps.2012.10.003</a>

Result language
angličtina
Original language name
Construction and characterization of a reporter gene cell line for assessment of human glucocorticoid receptor activation
Original language description
Glucocorticoids are widely used drugs in human pharmacotherapy. There is an increasing demand for tools allowing detection of the ligands for glucocorticoid receptor (GR), with regard to pre-clinical drug testing and environmental applications. We constructed human luciferase reporter gene cell line AZ-GR derived from HeLa human cervix carcinoma cells, which were stably transfected with reporter plasmid containing three copies of glucorticoid response element (GRE) upstream of luciferase reporter gene.We isolated five dexamethasone-responsive clones, and we further characterized two most responsive ones (AZ-GR). Dose-response analyses were performed with 22 different natural and synthetic steroids and the values of EC50 were calculated. AZ-GR cells displayed high specificity and sensitivity to glucocorticoids, very low responsiveness to mineralocorticoids, but no responsiveness to estrogens, gestagens or androgens. Time-course analyses revealed that AZ-GR cells allow detection of GR a
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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