Effects of Flavored Nonalcoholic Beverages on Transcriptional Activities of Nuclear and Steroid Hormone Receptors: Proof of Concept for Novel Reporter Cell Line PAZ-PPARg

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F18%3A73591319" target="_blank" >RIV/61989592:15310/18:73591319 - isvavai.cz</a>
Result on the web
<a href="https://pubs.acs.org/doi/10.1021/acs.jafc.8b05158" target="_blank" >https://pubs.acs.org/doi/10.1021/acs.jafc.8b05158</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.jafc.8b05158" target="_blank" >10.1021/acs.jafc.8b05158</a>

Result language
angličtina
Original language name
Effects of Flavored Nonalcoholic Beverages on Transcriptional Activities of Nuclear and Steroid Hormone Receptors: Proof of Concept for Novel Reporter Cell Line PAZ-PPARg
Original language description
We developed and characterized a novel human luciferase reporter cell line for the assessment of peroxisome proliferator-activated receptor gamma (PPAR gamma) transcriptional activity, PAZ-PPARg. The luciferase activity induced by PPAR gamma endogenous agonist 15d-PGJ2 and prostaglandin PGD2 reached mean values of (87.9 +/- 14.0)-fold and (89.6 +/- 19.7)-fold after 24 h of exposure to 40 mu M 15d-PGJ2 and 70 mu M PGD2, respectively. A concentration-dependent inhibition of 15d-PGJ2- and PGD2-induced luciferase activity was observed after the application of T0070907, a selective antagonist of PPAR gamma, which confirms the specificity of response to both agonists. The PAZ-PPARg cell line, along with the reporter cell lines for the assessment of transcriptional activities of thyroid receptor (TR), vitamin D3 receptor (VDR), androgen receptor (AR), and glucocorticoid receptor (GR), were used for the screening of 27 commonly marketed flavored nonalcoholic beverages for their possible disrupting effects. Our findings indicate that some of the examined beverages have the potential to modulate the transcriptional activities of PPAR gamma, VDR, and AR.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30108 - Toxicology

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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