Role of chloride ion in hydroxyl radical production in photosystem II under heat stress: Electron paramagnetic resonance spin-trapping study

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33142585" target="_blank" >RIV/61989592:15310/12:33142585 - isvavai.cz</a>
Result on the web
<a href="http://download.springer.com/static/pdf/56/art%253A10.1007%252Fs10863-012-9433-4.pdf?auth66=1362665862_679ae7727f40de6fc139c6eddcaa1261&ext=.pdf" target="_blank" >http://download.springer.com/static/pdf/56/art%253A10.1007%252Fs10863-012-9433-4.pdf?auth66=1362665862_679ae7727f40de6fc139c6eddcaa1261&ext=.pdf</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10863-012-9433-4" target="_blank" >10.1007/s10863-012-9433-4</a>

Result language
angličtina
Original language name
Role of chloride ion in hydroxyl radical production in photosystem II under heat stress: Electron paramagnetic resonance spin-trapping study
Original language description
Hydroxyl radical (HO?) generation in photosystem II (PSII) was studied by electron paramagnetic resonance (EPR) spin-trapping technique. It is demonstrated here that the exposure of PSII membranes to heat stress (40 °C) results in HO? formation, as monitored by the formation of EMPO-OH adduct EPR signal. The presence of different exogenous halides significantly suppressed the EMPO-OH adduct EPR signal in PSII membranes under heat stress. The addition of exogenous acetate and blocker of chloride channelsuppressed EMPO-OH adduct EPR signal, whereas the blocker of calcium channel did not affect EMPO-OH adduct EPR signal. Heat-induced hydrogen peroxide (H2O2) production was studied by amplex red fluorescent assay. The presence of exogenous halides, acetate and chloride blocker showed the suppression of H2O2 production in PSII membranes under heat stress. Based on our results, it is proposed that the formation of HO? under heat stress is linked to uncontrolled accessibility of water to the
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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