Dark production of reactive oxygen species in photosystem II membrane particles at elevated temperature - EPR spin-trapping study.
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F07%3A00004659" target="_blank" >RIV/61989592:15310/07:00004659 - isvavai.cz</a>
Result on the web
—
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Dark production of reactive oxygen species in photosystem II membrane particles at elevated temperature - EPR spin-trapping study.
Original language description
In our study, spin-trapping EPR technique was employed to study dark production of two reactive oxygen species, hydroxyl radicals (OH?) and singlet oxygen (1O2), in spinach PSII membrane particles exposed to elevated temperature (47 °C). Production of OH?, evaluated as EMPO-OH adduct EPR signal, was suppressed by the enzymatic removal of hydrogen peroxide and by the addition of iron chelator desferal, whereas externally added hydrogen peroxide enhanced OH? production. These observations reveal that OH?is presumably produced by metal-mediated reduction of hydrogen peroxide in a Fenton-type reaction. Increase in pH above physiological values significantly stimulated the formation of OH?, whereas the presence of chloride and calcium ions had the oppositeeffect. Based on our results it is proposed that the formation of OH? is linked to the thermal disassembly of water-splitting manganese complex on PSII donor side.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
—
Result continuities
Project
<a href="/en/project/1K04103" target="_blank" >1K04103: Reactive oxygen species in photosynthesis</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2007
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biochimica Et Biophysica Acta - Bioenergetics
ISSN
0005-2728
e-ISSN
—
Volume of the periodical
1767
Issue of the periodical within the volume
6
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
—
UT code for WoS article
—
EID of the result in the Scopus database
—