Molecular Mechanism of preQ(1) Riboswitch Action: A Molecular Dynamics Study

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F12%3A33142847" target="_blank" >RIV/61989592:15310/12:33142847 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/12:00389524 RIV/00216224:14740/12:00063769
Result on the web
<a href="http://dx.doi.org/10.1021/jp309230v" target="_blank" >http://dx.doi.org/10.1021/jp309230v</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/jp309230v" target="_blank" >10.1021/jp309230v</a>

Result language
angličtina
Original language name
Molecular Mechanism of preQ(1) Riboswitch Action: A Molecular Dynamics Study
Original language description
Riboswitches often occur in the 5'-untranslated regions of bacterial mRNA where they regulate gene expression. The preQ(1) riboswitch controls the biosynthesis of a hyper-modified nucleoside queuosine in response to binding the queuosine metabolic intermediate. Structures of the ligand-bound and ligand-free states of the preQ(1) riboswitch from Thermoanaerobacter tengcongensis were determined recently by Xray crystallography. We used multiple, microsecond-long molecular dynamics simulations (29 mu s intotal) to characterize the structural dynamics of preQ(1) riboswitches in both states. We observed different stabilities of the stem in the bound and free states, resulting in different accessibilities of the ribosomebinding site. These differences are related to different stacking interactions between nucleotides of the stem and the associated loop, which itself adopts different conformations in the bound and free states. We suggest that the loop not only serves to bind preQ, but also t
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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