LC/MS metabolic study on quercetin and taxifolin galloyl esters using human hepatocytes as toxicity and biotransformation in vitro cell model

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F13%3A33144017" target="_blank" >RIV/61989592:15310/13:33144017 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/13:00424179 RIV/61989592:15110/13:33144017
Result on the web
<a href="http://dx.doi.org/10.1016/j.jpba.2013.07.045" target="_blank" >http://dx.doi.org/10.1016/j.jpba.2013.07.045</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jpba.2013.07.045" target="_blank" >10.1016/j.jpba.2013.07.045</a>

Result language
angličtina
Original language name
LC/MS metabolic study on quercetin and taxifolin galloyl esters using human hepatocytes as toxicity and biotransformation in vitro cell model
Original language description
Galloyl esters of quercetin and taxifolin have been recently prepared semisynthetically as part of work towards modifying the solubility and modulating the biological aktivity of these natural flavonoids. In this paper we focused on the liquid chromatography?mass spektrometry (LC?MS) profiling of metabolites of 3-O-galloylquercetin and 7-O-galloyltaxifolin using human hepatocytes as the in vitro cell model. A subtoxic concentration (50 M) was used for both compounds and the formation of metabolites wasmonitored for 2 h in hepatocytes and cultivation medium separately. Using negative electrospray ionization-quadrupole time-of-flight mass spektrometry (ESI-QqTOF MS), we identified different biotransformation patterns for the studied compounds. 3-O-Galloylquercetin is metabolized directly to glucuronides and methyl derivatives. In contrast, 7-O-galloyltaxifolin is oxidized to 7-O-galloylquercetin or cleaved to taxifolin, and consequently the products formed are sulfated or glucuronidated
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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