Metabolism of palmatine by human hepatocytes and recombinant cytochromes P450

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15310%2F15%3A33149675" target="_blank" >RIV/61989592:15310/15:33149675 - isvavai.cz</a>
Alternative codes found
RIV/61989592:15110/15:33149675
Result on the web
<a href="http://www.sciencedirect.com/science/article/pii/S073170851400452X" target="_blank" >http://www.sciencedirect.com/science/article/pii/S073170851400452X</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jpba.2014.09.015" target="_blank" >10.1016/j.jpba.2014.09.015</a>

Result language
angličtina
Original language name
Metabolism of palmatine by human hepatocytes and recombinant cytochromes P450
Original language description
In this study, we developed a new liquid chromatography-mass spectrometry (LC-MS) method for analysis of the protoberberine alkaloid palmatine and its metabolites with separation performed on acyanopropyl-modified stationary phase. Palmatine was metabolized using suspensions of human hepatocytes and human recombinant cytochrome P450 (CYP) enzymes. Our analyses using electrospray ionization-quadrupole time-of-flight mass spectrometry revealed that palmatine was relatively resistant to the metabolic activity of human hepatocytes and recombinant CYP enzymes. However, we found that the biotransformation of palmatine in human hepatocytes included O-demethylation or hydroxylation, and that the product of palmatine demethylation was conjugated by glucuronidation or sulfation. Moreover, we found that human recombinant CYP2D6 and, to a lesser extent, CYP1A2 can mediate O-demethylation of palmatine. These results provide fundamental insights into the biotransformation of palmatine in human in vi
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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